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Título : Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia.
Autor : Rendón, Julio César
Cortés Mancera, Fabián
Restrepo Gutiérrez, Juan Carlos
Hoyos, Sergio
Navas, María Cristina
Palabras clave : Hepatitis B
Liver diseases
End Stage Liver Disease
Genome, Viral
Hepatitis B Surface Antigens
Liver Transplantation
Liver inflammation
Risk Factors
Viral Load
Fecha de publicación : 2017
Editorial : Public Library of Science
Citación : Rendón JC, Cortés-Mancera F, Restrepo-Gutiérrez JC, Hoyos S, Navas MC. Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia. PLoS One. 2017 Jul 7;12(7):e0180447.
Abstract : BACKGROUND: Hepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease. METHODS: Sixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique. RESULTS: In five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X. CONCLUSIONS: This is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis.
Grupo de INV. : Grupo de Gastrohepatología
ISSN : 19326203
Aparece en las colecciones: Instituto de Investigaciones Médicas

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